Minimal Sequence Requirements for Synthetic Peptides Derived from the V3 Loop of the Human Immunodeficiency Virus Type 1 (HIV-1) to Enhance HIV-1 Binding to Cells and Infection
Identifieur interne : 004158 ( Main/Exploration ); précédent : 004157; suivant : 004159Minimal Sequence Requirements for Synthetic Peptides Derived from the V3 Loop of the Human Immunodeficiency Virus Type 1 (HIV-1) to Enhance HIV-1 Binding to Cells and Infection
Auteurs : Carlo Zanotto [Italie] ; Francesca Calderazzo [Italie] ; Monica Dettin [Italie] ; Carlo Di Bello [Italie] ; Monica Autiero [Italie] ; John Guardiola [Italie] ; Luigi Chieco-Bianchi [Italie] ; Anita De Rossi [Italie]Source :
- Virology [ 0042-6822 ] ; 1995.
Abstract
Abstract: We previously demonstrated that a 23-mer peptide (DB3) derived from the V3 loop of the surface glycoprotein of HIV-1 MN strain was able to bind to soluble CD4 and enhance HIV-1 infection. The mechanism and structural features required for these biological activities were studied by using shortened DB3 derivatives and DB3 analogs carrying single amino acid substitutions. We found that peptides in which the aromatic amino acid in position 15 or 16 had been replaced by an uncharged hydrophobic residue (DB3-115 and DB3-116), analogs in which positively charged amino acids were replaced by corresponding D-enantiomers, and shortened DB3-derivatives lost both enhancing activity and ability to bind to soluble CD4. Other peptide variants in which a positively charged amino acid was replaced by asparagine at positions 3 (DB3-N3), 6 (DB3-N6), and 19 (DB3-N19), respectively, retained both enhancing and binding activities, although with different efficiencies. The CD4 binder peptides DB3 and DB3-N19, but none of the CD4 nonbinder peptides, enhanced CD4 expression on peptidetreated cells as well as gp120 binding to both CD4+ cells and soluble CD4. These findings strongly suggest that the peptide/CD4 interaction induced an increase in both CD4 expression and CD4/gp120 binding affinity, which in turn mediated the enhancement of viral infection. A model of the structural conformation of DB3 peptide required for its biological activities is discussed.
Url:
DOI: 10.1006/viro.1995.1003
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002039
- to stream Istex, to step Curation: 002039
- to stream Istex, to step Checkpoint: 001849
- to stream Main, to step Merge: 004219
- to stream Main, to step Curation: 004158
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Minimal Sequence Requirements for Synthetic Peptides Derived from the V3 Loop of the Human Immunodeficiency Virus Type 1 (HIV-1) to Enhance HIV-1 Binding to Cells and Infection</title><author><name sortKey="Zanotto, Carlo" sort="Zanotto, Carlo" uniqKey="Zanotto C" first="Carlo" last="Zanotto">Carlo Zanotto</name></author><author><name sortKey="Calderazzo, Francesca" sort="Calderazzo, Francesca" uniqKey="Calderazzo F" first="Francesca" last="Calderazzo">Francesca Calderazzo</name></author><author><name sortKey="Dettin, Monica" sort="Dettin, Monica" uniqKey="Dettin M" first="Monica" last="Dettin">Monica Dettin</name></author><author><name sortKey="Di Bello, Carlo" sort="Di Bello, Carlo" uniqKey="Di Bello C" first="Carlo" last="Di Bello">Carlo Di Bello</name></author><author><name sortKey="Autiero, Monica" sort="Autiero, Monica" uniqKey="Autiero M" first="Monica" last="Autiero">Monica Autiero</name></author><author><name sortKey="Guardiola, John" sort="Guardiola, John" uniqKey="Guardiola J" first="John" last="Guardiola">John Guardiola</name></author><author><name sortKey="Chieco Bianchi, Luigi" sort="Chieco Bianchi, Luigi" uniqKey="Chieco Bianchi L" first="Luigi" last="Chieco-Bianchi">Luigi Chieco-Bianchi</name></author><author><name sortKey="De Rossi, Anita" sort="De Rossi, Anita" uniqKey="De Rossi A" first="Anita" last="De Rossi">Anita De Rossi</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:9E9CC66AA46BEF55D17004CCABB2DE486187AE92</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1006/viro.1995.1003</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-3BG2VZJ4-4/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002039</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002039</idno><idno type="wicri:Area/Istex/Curation">002039</idno><idno type="wicri:Area/Istex/Checkpoint">001849</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001849</idno><idno type="wicri:doubleKey">0042-6822:1995:Zanotto C:minimal:sequence:requirements</idno><idno type="wicri:Area/Main/Merge">004219</idno><idno type="wicri:Area/Main/Curation">004158</idno><idno type="wicri:Area/Main/Exploration">004158</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Minimal Sequence Requirements for Synthetic Peptides Derived from the V3 Loop of the Human Immunodeficiency Virus Type 1 (HIV-1) to Enhance HIV-1 Binding to Cells and Infection</title><author><name sortKey="Zanotto, Carlo" sort="Zanotto, Carlo" uniqKey="Zanotto C" first="Carlo" last="Zanotto">Carlo Zanotto</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Oncology, Interuniversity Center for Cancer Research, Padova, Italy; Institute of Industrial Chemistry, University of Padova, Padova, Italy; and International Institute of Genetics and Biophysics, CNR, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Calderazzo, Francesca" sort="Calderazzo, Francesca" uniqKey="Calderazzo F" first="Francesca" last="Calderazzo">Francesca Calderazzo</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Oncology, Interuniversity Center for Cancer Research, Padova, Italy; Institute of Industrial Chemistry, University of Padova, Padova, Italy; and International Institute of Genetics and Biophysics, CNR, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Dettin, Monica" sort="Dettin, Monica" uniqKey="Dettin M" first="Monica" last="Dettin">Monica Dettin</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Oncology, Interuniversity Center for Cancer Research, Padova, Italy; Institute of Industrial Chemistry, University of Padova, Padova, Italy; and International Institute of Genetics and Biophysics, CNR, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Di Bello, Carlo" sort="Di Bello, Carlo" uniqKey="Di Bello C" first="Carlo" last="Di Bello">Carlo Di Bello</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Oncology, Interuniversity Center for Cancer Research, Padova, Italy; Institute of Industrial Chemistry, University of Padova, Padova, Italy; and International Institute of Genetics and Biophysics, CNR, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Autiero, Monica" sort="Autiero, Monica" uniqKey="Autiero M" first="Monica" last="Autiero">Monica Autiero</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Oncology, Interuniversity Center for Cancer Research, Padova, Italy; Institute of Industrial Chemistry, University of Padova, Padova, Italy; and International Institute of Genetics and Biophysics, CNR, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Guardiola, John" sort="Guardiola, John" uniqKey="Guardiola J" first="John" last="Guardiola">John Guardiola</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Oncology, Interuniversity Center for Cancer Research, Padova, Italy; Institute of Industrial Chemistry, University of Padova, Padova, Italy; and International Institute of Genetics and Biophysics, CNR, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Chieco Bianchi, Luigi" sort="Chieco Bianchi, Luigi" uniqKey="Chieco Bianchi L" first="Luigi" last="Chieco-Bianchi">Luigi Chieco-Bianchi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Oncology, Interuniversity Center for Cancer Research, Padova, Italy; Institute of Industrial Chemistry, University of Padova, Padova, Italy; and International Institute of Genetics and Biophysics, CNR, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="De Rossi, Anita" sort="De Rossi, Anita" uniqKey="De Rossi A" first="Anita" last="De Rossi">Anita De Rossi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Oncology, Interuniversity Center for Cancer Research, Padova, Italy; Institute of Industrial Chemistry, University of Padova, Padova, Italy; and International Institute of Genetics and Biophysics, CNR, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">206</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="807">807</biblScope><biblScope unit="page" to="816">816</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We previously demonstrated that a 23-mer peptide (DB3) derived from the V3 loop of the surface glycoprotein of HIV-1 MN strain was able to bind to soluble CD4 and enhance HIV-1 infection. The mechanism and structural features required for these biological activities were studied by using shortened DB3 derivatives and DB3 analogs carrying single amino acid substitutions. We found that peptides in which the aromatic amino acid in position 15 or 16 had been replaced by an uncharged hydrophobic residue (DB3-115 and DB3-116), analogs in which positively charged amino acids were replaced by corresponding D-enantiomers, and shortened DB3-derivatives lost both enhancing activity and ability to bind to soluble CD4. Other peptide variants in which a positively charged amino acid was replaced by asparagine at positions 3 (DB3-N3), 6 (DB3-N6), and 19 (DB3-N19), respectively, retained both enhancing and binding activities, although with different efficiencies. The CD4 binder peptides DB3 and DB3-N19, but none of the CD4 nonbinder peptides, enhanced CD4 expression on peptidetreated cells as well as gp120 binding to both CD4+ cells and soluble CD4. These findings strongly suggest that the peptide/CD4 interaction induced an increase in both CD4 expression and CD4/gp120 binding affinity, which in turn mediated the enhancement of viral infection. A model of the structural conformation of DB3 peptide required for its biological activities is discussed.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Zanotto, Carlo" sort="Zanotto, Carlo" uniqKey="Zanotto C" first="Carlo" last="Zanotto">Carlo Zanotto</name></noRegion><name sortKey="Autiero, Monica" sort="Autiero, Monica" uniqKey="Autiero M" first="Monica" last="Autiero">Monica Autiero</name><name sortKey="Calderazzo, Francesca" sort="Calderazzo, Francesca" uniqKey="Calderazzo F" first="Francesca" last="Calderazzo">Francesca Calderazzo</name><name sortKey="Chieco Bianchi, Luigi" sort="Chieco Bianchi, Luigi" uniqKey="Chieco Bianchi L" first="Luigi" last="Chieco-Bianchi">Luigi Chieco-Bianchi</name><name sortKey="De Rossi, Anita" sort="De Rossi, Anita" uniqKey="De Rossi A" first="Anita" last="De Rossi">Anita De Rossi</name><name sortKey="Dettin, Monica" sort="Dettin, Monica" uniqKey="Dettin M" first="Monica" last="Dettin">Monica Dettin</name><name sortKey="Di Bello, Carlo" sort="Di Bello, Carlo" uniqKey="Di Bello C" first="Carlo" last="Di Bello">Carlo Di Bello</name><name sortKey="Guardiola, John" sort="Guardiola, John" uniqKey="Guardiola J" first="John" last="Guardiola">John Guardiola</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004158 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004158 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:9E9CC66AA46BEF55D17004CCABB2DE486187AE92
|texte= Minimal Sequence Requirements for Synthetic Peptides Derived from the V3 Loop of the Human Immunodeficiency Virus Type 1 (HIV-1) to Enhance HIV-1 Binding to Cells and Infection
}}
| This area was generated with Dilib version V0.6.33. |  |